Immunologische Wirkungen von Citrus e fructibus und Cydonia e fructibus als Einzelarzneimittel und als Kombinationspräparat in vitro bei der saisonalen allergischen Rhinitis
Der Merkurstab 2015;68(1):9-19.
Artikel-ID: DMS-20424-DE
DOI: https://doi.org/10.14271/DMS-20424-DE
Die Arbeit untersucht die immunologischen Wirkungen des Kombinationspräparats Citrus e fructibus/Cydonia e fructibus (Citrus/Cydonia; jeweils 0,01 g/ml Citrus und 0,01 g/ml Cydonia) sowie der Einzelpräparate Citrus e fructibus (Citrus, 0,01 g/ml) und Cydonia e fructibus (Cydonia, 0,01 g/ml) auf die saisonale allergische Rhinitis (SAR). Mononukleäre Zellen des peripheren Blutes (PBMC) von fünf gesunden Spendern und fünf Spendern mit Gräserpollen-Allergie wurden isoliert und nach polyklonaler und allergenspezifischer Stimulation von T-Zellen in Gegenwart der drei Extrakte in vitro analysiert. Die Analysen zeigten eine Zellüberlebensrate im üblichen Bereich ohne Hinweise auf toxische Wirkungen. Citrus reduzierte vornehmlich die allergenspezifische chronisch-entzündliche Aktivität [TNF-α; Citrus versus Cydonia und Citrus/Cydonia: –87,4 (p < 0,001) bzw. –68,0 (p < 0,05)] und Aktivität des Th2-Wegs [IL-5; Citrus versus Cydonia: –217,8 (p < 0,01)], wohingegen sowohl Cydonia als auch Citrus/Cydonia im Wesentlichen den allergenspezifischen Th1-Weg induzierten [IFN-γ; Cydonia und Citrus/Cydonia versus Citrus: 3,8 (p < 0,01) bzw. 3,0 (p < 0,01)]. Citrus und Cydonia wiesen bei der Behandlung der SAR unterschiedliche Wirk mechanismen auf, das Kombinationspräparat war nicht wirksamer als die Einzelpräparate. Es sind weitere vergleichende Untersuchungen zum Nutzen und zur Wirksamkeit der Präparate in vivo notwendig.
A comparative in vitro study of the effects of separate and combined products of Citrus e fructibus and Cydonia e fructibus on immunological parameters of seasonal allergic rhinitis
This paper examined the effects of the combined product, Citrus e fructibus/Cydonia e fructibus (Citrus/Cydonia; Citrus and Cydonia: each 0,01 g/ml), and separate products of Citrus (0,01 g/ml) and Cydonia (0,01 g/ml) on the immunological pathways involved in seasonal allergic rhinitis (SAR). Peripheral blood mononuclear cells (PBMCs) from five healthy and five grass pollen-allergic donors were isolated and analyzed in vitro after polyclonal and allergen-specific stimulation of T cells in the presence of the three extracts. The analyses demonstrated acceptable cell survival with no signs of toxicity. Citrus mainly had a selective effect on reducing allergen-specific chronic inflammatory [TNF-α; Citrus compared to Cydonia and Citrus/Cydonia: –87.4 (p < 0,001) and –68.0 (p < 0,05), resp.] and Th2 pathway activity [IL-5; Citrus compared to Cydonia: –217.8 (p < 0,01)]; while, both Cydonia and Citrus/Cydonia mainly affected the induction of the allergen- specific Th1 pathway [IFN-γ; Cydonia and Citrus/Cydonia compared to Citrus: 3.8 (p < 0,01) and 3.0 (p < 0,01), resp.]. Citrus and Cydonia demonstrated different working mechanisms in the treatment of SAR and the combination product did not demonstrate larger effects than the separate preparations. Further effectiveness and efficacy studies comparing the effects of the products on SAR in vivo are indicated.
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